Dipstick enzyme immunoassay to detect Fusarium T-2 toxin in wheat

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Metabolism of the Fusarium Mycotoxins T-2 Toxin and HT-2 Toxin in Wheat

To investigate the metabolic fate of HT-2 toxin (HT2) and T-2 toxin (T2) in wheat (Triticum aestivum L.), an untargeted metabolomics study utilizing stable isotopic labeling and liquid chromatography-high resolution mass spectrometry was performed. In total, 11 HT2 and 12 T2 derived in planta biotransformation products were annotated putatively. In addition to previously reported mono- and digl...

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An Investigation on T-2 Toxin Contamination in Wheat Crops from 11 Cities, Iran

Objective: The major Fusarium toxins found in cereals and cereal-based products that can be harmful to both human and animal health are some trichothecenes, such as deoxynivalenol (DON). Methods: A recent SCOOP project, Since toxinogenic fungi are cosmopolitan, mycotoxins are environmental pollutants present in virtually all parts of the world and causing diseases (mycotoxicoses) . Thi...

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An Investigation on T-2 Toxin Contamination in Wheat Crops from 11 Cities, Iran

Objective: The major Fusarium toxins found in cereals and cereal-based products that can be harmful to both human and animal health are some trichothecenes, such as deoxynivalenol (DON). Methods: A recent SCOOP project, Since toxinogenic fungi are cosmopolitan, mycotoxins are environmental pollutants present in virtually all parts of the world and causing diseases (mycotoxicoses) . Thi...

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Dipstick immunoassay to detect enterohemorrhagic Escherichia coli O157:H7 in retail ground beef.

A sensitive and easy-to-perform dipstick immunoassay to detect Escherichia coli O157:H7 in retail ground beef was developed by using a sandwich-type assay (with a polyclonal antibody to E. coli O157 as the capture antibody and a monoclonal antibody to E. coli O157:H7 as the detection antibody) on a hydrophobic polyvinylidine difluoride-based membrane. E. coli O157:H7 in ground beef could be det...

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Biosynthesis of radiolabeled T-2 toxin by Fusarium tricinctum.

Incubation of Fusarium tricinctum NRRL 3299 on a solid rice medium in the presence of [1-14C]sodium acetate, [2-3H]mevalonic acid, [2-14C]mevalonic acid, or [5-3H]mevalonic acid yielded preparations of radiolabeled T-2 toxin with specific activities of 1.008, 1.64, 0.656, and 7.35 muCi/mmol, respectively.

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ژورنال

عنوان ژورنال: Applied and Environmental Microbiology

سال: 1996

ISSN: 0099-2240,1098-5336

DOI: 10.1128/aem.62.6.1880-1884.1996